ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (10): 2115-2125.doi: 10.11843/j.issn.0366-6964.2016.10.021

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Preparation and Content Measurement of Oxyclozanide Oral Suspension

ZHANG Ji-li,LI Bing,SI Hong-fei,GUO Xiao,ZHU Zhen,SHANG Xiao-fei,ZHOU Xu-zheng,ZHANG Ji-yu*   

  1. (Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture,Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China)
  • Received:2016-02-01 Online:2016-10-23 Published:2016-10-23

Abstract:

The study aimed to prepare oxyclozanide oral suspension and evaluate its physical and chemical properties,and establish an HPLC method for the determination of oxyclozanide.The sedimentation volume ratio and redispersibility were used as variable factors,the single factor experiment and the quadratic regression orthogonal rotational combination design were carried out to optimize the prescription.Then,the shape,size of oxyclozanide suspension was measured by transmission electron microscopylaser and particle size analyzer,and the content of oxyclozanide in suspension was determined by HPLC.The result showed that the suspension (100 mL) was consisted of 3.2 g oxyclozanide,0.2 g carbomer 974p,0.3 g sodium dodecyl sulfate sodium salt,0.02 g methyl 4-hydroxybenzoate and 0.4 g sodium pyrosulfite.With good redispersibility,the sedimentation volume ratio of oxyclozanide suspension was 0.999.Excellent line relationship was obtained in the range of 18-100 μg•mL-1 (r2=0.999 3).The oxyclozanide suspension was successfully prepared with simple preparation process,good stability and well quality-controlled product.

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